How to Build Plasmid Engineering Workbench with Circular Mapping, Restriction Analysis, Virtual Gels, and Primer Design

A Google Colab-native plasmid workbench is built using Biopython, NumPy, and Matplotlib to recreate the core ideas of SpliceCraft in an interactive notebook environment. The workbench loads plasmid records, normalizes annotated genomic features, renders circular and linear plasmid maps, computes sequence statistics, and analyzes restriction enzyme cut sites, among other functions. It uses a synthetic offline plasmid for reliable execution without external dependencies.